Ultrastructural changes in myocardial anoxia and reoxygenation
Bugiardini, R.; Vinelli, S.; Bubola, G.; Pettazzoni, P.; Puddu, P.
Bollettino della Societa Italiana di Biologia Sperimentale 58(1-2): 8-14
1982
ISSN/ISBN: 0037-8771 PMID: 7066103 Document Number: 195345
The aim of this study is to describe the ultrastructural changes in anoxic rat hearts perfused with Langendorff technique and the effects of reoxygenation on myocardial cells. After 1 h of aerobic perfusion, the hearts were subjected to 30 min of anoxia and then to 5 and 30 min of reoxygenation. The following findings were observed: after 30 min of anoxia, loss of mitochondrial matrix and dilatation of the intracristal spaces. After 5 min of reoxygenation: diffuse mitochondrial swelling, sarcomeres disarranged and out of register, several contraction bands. After 30 min: no evidence of cytoplasmic or mitochondrial swelling. The maximum enzyme release was observed when a wide myofibrillar contraction occurred. These results may suggest that the oxygen availability consequent to myocardium anoxia lead to further cell damage.