Proliferation of human acute leukemia cells in culture on treatment with an homo-aza-steroid
Politis, G.; Camoutsis, C.; Catsoulacos, P.
Methods and Findings in Experimental and Clinical Pharmacology 4(6): 403-406
1982
ISSN/ISBN: 0379-0355 PMID: 7144333 Document Number: 195094
When cultures of human leukemia cells from 9 untreated patients with acute leukemia were treated with 3.beta.-hydroxy-13.alpha.-amino-13,17-seco-5.alpha.-androstan-17-oic-13,17-lactam, an increased proliferating activity was exhibited. The leukemia cells were cultured for 48 h and the amounts of lactam (4, 12 and 24 ng/ml) were added at zero time in the continuous presence of (3H)-thymidine. Apparently, in high concentrations lactam acts as a toxic agent, while in the lower ones it seems to stimulate proliferation of the blast cell.