Reassociation kinetics of the DNA from rat liver nuclei of varying ploidy
Lebedeva, G.S.; Diment, A.V.
Tsitologiia 24(2): 219-223
1982
ISSN/ISBN: 0041-3771 PMID: 7071940 Document Number: 194301
Using sucrose gradient centrifugation, isolated liver cell nuclei were fractionated according to different ploidy classes. DNA was isolated from the di- and polyploid as well as from the total nuclear fractions, and the kinetics of their reassociation was investigated. A similar mode of reassociation was revealed for DNAs isolated from the nuclei of different ploidy classes, testifying in favour of the true polyploidy of liver cell nuclei.
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