Islet cell analysis and purification by light scatter and autofluorescence
Van de Winkle, M.; Maes, E.; Pipeleers, D.
Biochemical and Biophysical Research Communications 107(2): 525-532
1982
ISSN/ISBN: 0006-291X PMID: 6751329 Document Number: 194148
Rat pancreatic A- and B-cells differ in light scatter and FAD-related fluorescence and are thus represented by 2 easily distinguishable populations in a fluorescence-activated cell sorter (FACS). Sorting of dissociated islet cells yields highly purified single A- and B-cell preparations. FACS-analysis of islet cells also indicated that FAD-fluorescence in B-cells is reduced within a 5 min exposure to 20 mM glucose; no variations were observed in A-cell fluorescence nor with 3-O-methylglucose or fructose. FACS-analysis of blood cells and of dissociated liver, parotid, pituitary and pancreatic exocrine cells demonstrated a wide variation in the respective FAD-fluorescence intensities, which could be used for their purification as viable single cells as well as in studying their metabolic redox state.