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Pyridoxamine-alpha-keto acid transamination activity of aspartate apoaminotransferases

Yagi, T.; Kagamiyama, H.; Nozaki, M.

Biochemical and Biophysical Research Communications 107(3): 897-902

1982

ISSN/ISBN: 0006-291X
PMID: 6753838
Document Number: 191224
Pyridoxamine-.alpha.-keto acid transamination activities of homogeneous aspartate apoaminotransferases from various organisms were determined. Aspartate apoaminotransferases from pig heart cytosol and bakers' yeast utilized both oxalacetate and .alpha.-ketoglutarate as amino acceptors, while those from pig heart mitochondria and bacteria (Escherichia coli B and Pseudomonas striata) showed reactivity only toward oxalacetate. Specific activities of bacterial aspartate apoaminotransferases were very high compared to those of the yeast and animal apoenzymes. Phosphate and various anions, including sulfate, raised the pyridoxamine-.alpha.-keto acid transamination activity of all the aspartate apoaminotransferases examined. However, a high concentration of phosphate inhibited the reaction.

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Khatra, B.S.; Chawla, R.K.; Wadsworth, A.D.; Rudman, D. 1977: Effect of dietary branched-chain alpha-keto acids on hepatic branched-chain alpha-keto acid dehydrogenase in the rat Journal of Nutrition 107(8): 1528-1536
Turano, C.; Riva, F.; Giartosio, A. 1982: Recent advances in the study of coenzyme binding to aspartate apoaminotransferases Advances in Experimental Medicine and Biology 148: 283-293
Iakushev, V.A.; Lifshits, R.I. 1971: Effect of aspartic acid on the spectrum and activity of transamination isoenzymes in the myocardium in the acute period of myocardial infarct Farmakologiia i Toksikologiia 34(6): 682-685
Fuchs, M.; Gerlach, J.; Unger, J.; Encke, J.; Smith, M.; Neuhaus, P.; Nündel, M.; Riedel, E. 1994: Alpha-keto acid metabolism by hepatocytes cultured in a hybrid liver support bioreactor International Journal of Artificial Organs 17(10): 554-558
Kovaleva, G.K.; Severin, E.S.; Fazella, R.; Khomutov, R.M. 1973: The mechanism of interaction between aspartate transaminase and threo-alpha-cycloglutamic acid (cis-4-amino-5-carboxymethylisoxazolidone-3) Biokhimiia 38(2): 365-374
Tumanian, V.G.; Shliapnikov, S.V.; Severin, E.S. 1980: Conformation of threo-alpha-cycloglutamic acid and pyridoxal-5'-phosphate aldimine in the active site of aspartate aminotransferase Molekuliarnaia Biologiia 14(3): 632-639
Marmo, E.; Somma, A.; Ottavo, R.; Vairo, G.; Spaziante, G.; DiMezza, F.; Paone, G. 1981: Effects of prostacyclin, 6-keto-PGF1 alpha PGF2 alpha and 15[S]15-methyl-PGF2 alpha on platelet aggregation in vitro Journal of Medicine 12(2-3): 97-108
Noda, Y.; Ogata, K.; Mori, A. 2003: Antioxidant activities of novel alpha-lipoic acid derivatives: N-(6, 8-dimercaptooctanoyl)-2-aminoethanesulfonate- and N-(6, 8-dimercaptooctanoyl)-L-aspartate-zinc complex Research Communications in Molecular Pathology and Pharmacology 113-114: 133-147
Weber, C.; Beetens, J.; van de Wiele, R.; Beile, A.; Lohkamp, R.; de Clerck, F.; Höller, M. 1989: Determination of 6-keto-PGF1 alpha, TXB2, PGF2 alpha, PGE2, PGD2 in human blood: a comparison of RIA and GC-NICI-MS Progress in Clinical and Biological Research 301: 113-117
Oie, S.; Chiang, J. 1989: Alteration in the pharmacologic activity of alpha 1 adrenergic antagonists by alpha-1-acid glycoprotein Progress in Clinical and Biological Research 300: 235-238
Sonesson, A.; Bryn, K.; Jantzen, E.; Larsson, L. 1989: Gas chromatographic determination of (phosphorylated) 2-keto-3-deoxyoctonic acid, heptoses and glucosamine in bacterial lipopolysaccharides after treatment with hydrofluoric acid, methanolysis and trifluoroacetylation Journal of Chromatography 487(1): 1-7
Gao, J. 1989: Prostaglandin E2, F2 alpha,6-keto-prostaglandin F1 alpha and thromboxane B2 in the placenta of normotensive women and women with pregnancy induced hypertension Zhonghua Fu Chan Ke Za Zhi 24(6): 331
Tanaka, Y.; Yamamoto, T.; Matsumoto, T.; Sugiyama, Y. 1986: Detection of 6-keto-prostaglandin F1 alpha and thromboxane B2 in human placental tissues Nihon Sanka Fujinka Gakkai Zasshi 38(9): 1653-1654
Lewis, S.D.; Ng, A.S.; Lyle, E.A.; Mellott, M.J.; Appleby, S.D.; Brady, S.F.; Stauffer, K.J.; Sisko, J.T.; Mao, S.S.; Veber, D.F. 1995: Inhibition of thrombin by peptides containing Lysyl-alpha-keto carbonyl derivatives Thrombosis and Haemostasis 74(4): 1107-1112
Zhang, M.L.; Ou, Y.A. 1989: Studies on the interrelation of plasma 6-keto-prostaglandin F1 alpha, thromboxane B2 and diabetic microangiopathy Zhonghua Nei Ke Za Zhi 28(3): 142-144; 185-186
Maurin, N. 1986: Routine measurement of thromboxane B2 and the prostacyclin metabolite 6-keto-prostaglandin F1 alpha in plasma Arzneimittel-Forschung 36(8): 1174-1179
Qu, Y.Z.; Wang, Y.E.; Li, X.X. 1996: Effects of thiofedrine on platelet aggregation, thromboxane B2 and 6-keto-PGF1 alpha in rats Methods and Findings in Experimental and Clinical Pharmacology 18(5): 297-300
Takeda, M.; Takagi, T. 1980: Effects of a new bronchodilator, (alpha RS)-3-formamido-4-hydroxy-alpha-[[[(alpha RS)-p-methoxy-alpha-methylphenethyl] amino] methyl] benzyl alcohol fumarate dihydrate (BD 40A) on gastric acid secretion and gastrointestinal motility Nihon Yakurigaku Zasshi. Folia Pharmacologica Japonica 76(3): 185-191
Wang, H. 1990: Effects of corn pollen on the peroxides and 6-keto-PGF1 alpha/TXB2 in hyperlipidemic rabbits Zhonghua Xin Xue Guan Bing Za Zhi 18(6): 370-373
Cai, W. 1990: Changes in plasma TXB2 and 6-keto-PGF1 alpha and left atrial thrombosis in mitral stenosis Zhonghua Xin Xue Guan Bing Za Zhi 18(2): 98