Theoretical and mathematical conditions for use of linearization methods for saturation-type radioassay data
Shaw, W.
Clinical Chemistry 28(5): 1196-1197
1982
ISSN/ISBN: 0009-9147 PMID: 7074904 Document Number: 184280
The algebraic equivalency of nine different methods for the linearization of saturation-type radioassay data was previously demonstrated mathematically to hold under the assumption that the antibody is saturated with ligand. Although compete saturation with antibody never occurs, a good approximation to a saturation condition occurs when the product of the labeled ligand and the affinity constant is 100 or more. The mathematical basis for determining when methods for the linearization of saturation-type radioassay data are valid is derived from a development of the first-order mass action law applied to ligand-antibody reactions.