Effect of 6,9-deepoxy-6,9- (phenylimino) -delta 6,8-prostaglandin 1 (1) (U-60,257) , an inhibitor of leukotriene synthesis, on human neutrophil function
Smith, R.J.; Sun, F.F.; Bowman, B.J.; Iden, S.S.; Smith, H.W.; Mcguire, J.C.
Biochemical and Biophysical Research Communications 109(3): 943-949
1982
ISSN/ISBN: 0006-291X PMID: 6297495 Document Number: 183750
A23187, a Ca ionophore, stimulated a time-dependent generation of 5(S), 12(R)-dihydroxy-6,8,10,14-eicosatetraenoic acid (leukotriene B4), production of superoxide anion (O2) and release of granule-associated .beta.-glucuronidase and lysozyme by human neutrophils. Leukotriene B4 also elicited the selective release of granule enzymes from cytochalasin B-treated neutrophils. U-60,257, a recently identified inhibitor of leukotriene (LT) C4 and D4 synthesis, caused a dose-related (1-10 .mu.M) suppression of LTB4 production by A23187-activated neutrophils. Degranulation and O2 generation by neutrophils exposed to A23187 and the chemotactic oligopeptide, N-f-Met-Leu-Phe (FMLP), were also inhibited with U-60,257.