On-line measurement of catecholamine secretion
Green, D.J.; Perlman, R.L.
Analytical Biochemistry 110(2): 270-276
1981
ISSN/ISBN: 0003-2697 PMID: 7235213 Document Number: 177587
A method for the on-line measurement of catecholamine secretion from rat pheochromocytoma chromaffin cells is described. Isolated chromaffin cells are placed on a membrane filter and superfused. The perfusate is passed directly over an electrochemical detector, where secreted catecholamines are oxidized. The catecholamine content in the perfusate is measured and recorded as a continuous current output from the detector. This on-line monitor was used to study the time course of catecholamine secretion, the desensitization of the secretory process and the recovery of the secretory response to acetylcholine.