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Procedure for granulokinetic studies in the horse with chromium-51

Carakostas, M.C.; Moore, W.E.; Smith, J.E.

American Journal of Veterinary Research 42(4): 620-622

1981

ISSN/ISBN: 0002-9645
PMID: 7332121
Document Number: 174639
A procedure with chromium-51 (51Cr) as the cell label that maintains high-cell viability for studying granulocyte kinetics in horses is described. The procedure combines and modifies several methods for isolating leukocytes and granulocytes for use in the horse when a large volume of labeled cells is required. Also described is an improved technique for measuring granulocyte specific activity in large serial blood samples, using a Ficoll-sedimentation method. The procedure should be useful for determining granulocyte kinetics in the horse, the only major domestic species for which such data are not available.

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