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"in vitro" culture of the oviduct of women, rats and rabbits. Preliminary results

Menezo, Y.; Bremond, A.; Khatchadourian, C.; Orgiazzi, A.; Rudigoz, R.

Journal de Gynecologie Obstetrique et Biologie de la Reproduction 10(7): 651-654

1981

ISSN/ISBN: 0368-2315
PMID: 7334190
Document Number: 172474
A continuous flow perfusion system which has been improved for the culture of large pieces of tissue, e.g. epididymis and ovarian follicles was tested for the entire oviduct in women, rabbits and rats. The histological properties of the organ were maintained after a day or two of culture; but only in the presence of oestrogens. Progesterone seems to induce a weakness of epithelial cell attachment and these become scattered in the lumen. In women the cultures seemed to be more or less permanently contamined, probably due to commensals, because this problem did not occur with rat or rabbit oviducts.

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