[125I]-antigammaglobulin antibodies as universal reagents in radioimmunochemical methods of investigation
Isachenkov, V.A.; Nikiforova, G.P.; Avilov, V.V.; Nagornaia, L.V.
Biokhimiia 46(1): 75-84
1981
ISSN/ISBN: 0320-9725 PMID: 6166332 Document Number: 171263
A new indirect radioimmunochemical method based on the use of [125I]-anti-IgG-antibodies as universal detecting reagents is proposed. Its first step consists in the antibody binding to the antigen to be analyzed; the second step -- in immunoadsorption of the non-bound antibodies by water-insoluble sorbents prepared by chlorocarbonic acid isobutyl ester copolymerization of antigens with serum albumin or by immobilization of the antigen on Sepharose. The third step is the determination of the amount of sorbent-bound antibodies by means of [125I]-anti-IgG-antibodies. The method proposed was used for quantitative estimation of prolactin, somatotropin, lutropin, BB-isoenzyme of human creatine phosphokinase, testosterone and 5 alpha-dihydroxytestosterone. The method does not employ labelled antigens and is highly sensitive and highly specific.