Antigen depressant effect of glutaraldehyde for aortic heterografts with a valve, with special reference to a concentration right fit for the preservation of grafts
Okamura, K.; Chiba, C.; Iriyama, T.; Itoh, T.; Maeta, H.; Ijima, H.; Mitsui, T.; Hori, M.
Surgery 87(2): 170-176
1980
ISSN/ISBN: 0039-6060 PMID: 6766575 Document Number: 167777
A suitable concentration of glutaraldehyde for preservation of aortic heterografts with a valve was investigated from the view points of immunologic, histological and hemodynamic studies. A heterograft model was prepared using dogs and raccoon dogs in such a way that aortas with valves of raccoon dogs were implanted into the descending aortas of dogs. A pressure study at 2-12 mo. after implantation demonstrated the following results: there was no pressure gradient between the graft and recipient aorta in the fresh graft group, there was a slight pressure gradient of 11 .+-. 3 and 10 .+-. 3 mm Hg in the 0.05% and 0.15% glutaraldehyde-preserved groups, respectively and a marked pressure gradient of 49 .+-. 2 mm Hg in the 0.3% glutaraldehyde group. Angiography showed aneurysmal dilatation of implanted grafts in a fresh graft group, no demonstrable deformity in the 0.05% and 0.15% preserved grafts, and a marked stenosis and shrinkage of grafts in the 0.3% group. These changes were substantiated from macroscopic and histological observation. Immunologic analysis of recipient dog sera using techniques for antiraccoon-dog hemagglutinin, hemolysin and lymphocytotoxin revealed an obvious antigen depressant effect of glutaraldehyde, even in cases in which highly diluted solution (0.05%) was used. A low-concentration solution of glutaraldehyde (0.15% or less) is evidently the choice for treatment of heterografts.