Cellular basis for elevated IgA responses in C3H/HeJ mice
Kiyono, H.; Babb, J.L.; Michalek, S.M.; McGhee, J.R.
Journal of Immunology 125(2): 732-737
1980
ISSN/ISBN: 0022-1767 PMID: 6967087 Document Number: 165211
When lipid A nonresponsive C3H/HeJ mice were intubated with SRBC abrogated in vitro responses. When Peyer's patch cultures from normal or carrier-primed mice were immunized with TNP-SRBC, no in vitro immune responses occurred in either mouse strain unless the macrophage monokine, Interleukin 1 (Il 1), was added to the culture. C3H/HeJ PP cultures from carrier-primed mice yielded higher responses than C3H/HeN cultures in the presence of Il 1. Mesenteric lymph node (MLN) cultures from carrier-primed C3H/HeJ mice exhibited higher in vitro responses to TNP-SRBC than did C3H/HeN MLN cultures. When submitogenic concentrations of Con A were tested with SRBC-primed PP T cells in splenic B cell cultures, those containing C3H/HeJ PP T cells yielded enhanced in vitro responses to TNP-SRBC. Oral administration of T-dependent antigens to C3H/HeJ mice results in increased T helper cell induction in GALT and leads to higher IgA responses in this mouse strain.