The interaction of carrier-bound methotrexate with L1210 cells
Chu, B.C.; Whiteley, J.M.
Molecular Pharmacology 17(3): 382-387
1980
ISSN/ISBN: 0026-895X PMID: 6156401 Document Number: 164277
Methotrexate (MTX) covalently linked to 2 randomly chosen (.alpha.-chymotrypsinogen and bovine IgMTX-BSA or MTX-125I-BSA indicated that the MTX-BSA complex was not degraded on the external surface of the cell membrane, as 60-65% of both radioactive labels was found inside the cell lysate fraction with much of the iodinated material degraded to low MW fragments. The 125I marker remaining bound to the cell membrane was still present as a nondialyzable high MW molecule. Experiments employing MTX bound to Dextran T70, an ineffective agent, indicated only limited cellular interaction and low drug uptake.