Top Abstract Purchase Related
Home
>
Node
>
Subnode

DNA synthesis in isolated nuclei of Misgurnus fossilis loach embryos. I. Characteristics of the system

Mikhaĭlov, V.S.; Guliamov, D.B.

Molekuliarnaia Biologiia 14(5): 1151-1158

1980

ISSN/ISBN: 0026-8984
PMID: 7421821
Document Number: 163784
System for studying DNA synthesis in the isolated nuclei of loach Misgurnus fossilis embryo was described. Nuclei from 13--15 h embryo incorporated 2--3 pmoles of [3H]TMP or [3H]dCMP per microgram of DNA during 30 min of incubation in the reaction mixture. The incorporation was inhibited by ethidium bromide, actinomycin D, and N-ethylmaleimide. The incorporation was Mg2+- and ATP-dependent. Adenosine diphosphate at a concentration of 10 mM enhanced the label incorporation into DNA about twofold. Nicotinamide adenine dinucleotide, polyamines, heparin, and histones inhibited label incorporation into nuclear DNA.

Document emailed within 1 workday
Secure & encrypted payments

Goĭda, E.A.; Savchuk, L.F. 1984: Phospholipids and cholesterol in oocytes and embryos of the loach (Misgurnus fossilis) Ukrainskii Biokhimicheskii Zhurnal 56(6): 668-671
Stoika, R.S.; Kusen', S.I. 1978: Lactate dehydrogenase isoenzymes in loach (Misgurnus fossilis) tissues Biokhimiia 43(6): 988-995
Stoĭka, R.S.; Kusen', S.I. 1981: Immunochemical study of lactate dehydrogenase polymorphism in the loach Misgurnus fossilis Biokhimiia 46(9): 1633-1639
Mikhaĭlov, V.S.; Guliamov, D.B. 1981: Isolation and characterization of DNA polymerase alpha, beta and gamma from the cells of the loach (Misgurnus fossilis) Biokhimiia 46(9): 1539-1547
Kusen', S.I.; Pashkovskaia, I.S. 1973: Effect of amino acids, hormones and actinomycin D on the aminotransferase activity of unfertilized loach (Misgurnus fossilis) roe Ukrains'kyi Biokhimichnyi Zhurnal 45(5): 565-569
Hoida, O.A.; Kusen', S.I.; Mukalov, I.O. 1975: Study of phospholipids during embryogenesis of Misgurnus fossilis Ukrains'kyi Biokhimichnyi Zhurnal 47(3): 370-373
Arai, K. 2003: Genetics of the loach, Misgurnus anguillicaudatus: recent progress and perspective Folia Biologica 51 Suppl: 107-117
Pajor, W.J. 1977: The activity of some intracellular oxidative enzymes in the thyroid follicular epithelium of Carassius auratus and Misgurnus fossilis Folia Biologica 25(2): 223-227
Koen, I.M.; Zbarskiĭ, I.B. 1978: RNA transport from rat liver cell nuclei in vitro. Release of rapidly labeled RNA from isolated nuclei in a cell-free system in the presence of RNAse affectors Biokhimiia 43(12): 2115-2123
Tishchenko, L.I.; Miul'berg, A.A.; Ashmarin, I.P. 1971: Interrelations between histone acetylation and RNA synthesis in isolated nuclei Biokhimiia 36(3): 595-603
Mikhaĭlov, V.S.; Guliamov, D.B.; Peskin, A.V.; Filatova, L.S. 1983: DNA polymerase gamma in the cells of loach embryos localized in mitochondria Biokhimiia 48(6): 1012-1019
Iukhnovskaia, N.I.; Pashkovskaia, I.S.; Sologub, L.I.; Kukhar, G.F. 1982: Proteolytic activity in unfertilized eggs and embryos of the loach under the effect of insulin Ukrainskii Biokhimicheskii Zhurnal 54(2): 176-179
Ivashkiv, L.I.; Tselevych, M.V.; Boĭko, N.M.; Sanahurs'kyĭ, D.I. 2005: Variance analysis of Cd2+, Zn2+, and Mn2+ effect on membrane Na+,K(+)-ATPase activity of loach embryos Ukrains'kyi Biokhimichnyi Zhurnal 77(3): 44-48
Schandl, E.K. 1973: Stimulation of oligodeoxyribonucleotide synthesis by cytoplasmic factors in the isolated nuclei of leukemic cells Cancer Research 33(10): 2398-2401
Iavdochenko, O.I.; Kusen', S.I.; Sologub, L.I. 1977: Effect of insulin on activity of carbohydrate metabolism enzymes in loach embryos in early development Ukrains'kyi Biokhimichnyi Zhurnal 49(3): 85-88
Kogan, G.L.; Berman, A.E.; Mazurov, V.I. 1973: Collagen biosynthesis in a cell-free system in the presence of RNA isolated from the polysomes of chick embryos Biokhimiia 38(4): 872-874
Yazdi, E.; Györkey, F.; Busch, H.; Györkey, P. 1971: Biochemical study of nuclei isolated from normal lung and lung tumors. I. Isolation of nuclei and characterization of nuclear RNA Journal of the National Cancer Institute 47(1): 121-129
Bogoliubova, N.A.; Bogoliubova, I.O. 2009: Actin localization in the nuclei of two-cell mouse embryos Tsitologiia 51(8): 663-669
Veĭko, N.N.; Liapunova, N.A.; Kovalev, L.I.; Ershova, E.S.; Spitkovskiĭ, D.M. 2000: Proteins, tightly bound with a transcribed segment of rDNA in chromatin from isolated nuclei, nucleoids and nucleoproteins of human lymphocytes. Isolation and partial characteristics of candidate proteins Molekuliarnaia Biologiia 34(2): 285-291
Schmidtke, J.R.; Johnson, A.G. 1971: Regulation of the immune system by synthetic polynucleotides. I. Characteristics of adjuvant action on antibody synthesis Journal of Immunology 106(5): 1191-1200