Spontaneous antibody-secreting human blood lymphocytes detected with a protein a plaque assay
Kunori, T.; Broman, P.; Tan, M.
Clinical and Experimental Immunology 42(1): 162-166
1980
ISSN/ISBN: 0009-9104 PMID: 7006874 Document Number: 161236
Spontaneous antibody-secreting lymphocytes in human peripheral blood were detected and counted by a protein A plaque assay. The level of plaque-forming cells (PFC) in peripheral blood was significantly higher in patients with immune disorders (per 10(6) lymphocytes: IgG PFC, 2,654 +/- 684; IgA PFC, 1,727 +/- 327; IgM PFC, 403 +/- 92) than in normal controls (per 10(6) lymphocytes: IgG PFC, 327 +/- 56; IgA PFC 633 +/- 128; IgM PFC, 215 +/- 105). There was no direct correlation between the PFC numbers of different Ig classes.