Concentration of middle-sized molecules in the blood of children with chronic renal failure during treatment with short-term hemodialysis
Gudim, V.I.; Sigalla, P.; Ivanova, V.S.; Oldag, K.
Voprosy Meditsinskoi Khimii 26(6): 742-746
1980
ISSN/ISBN: 0042-8809 PMID: 7456404 Document Number: 160792
Blood serum from children and young patients with chronic kidney insufficiency at the terminal step as well as from healthy donors was fractionated using ultrafilters "Amicon" XM-50 and Sephadex G-25. Three fractions were obtained: F1--molecular mass 50,000-5,000, F3--3,000-800 (fraction of middle mass molecules, MM) and F5 < 200. Amount of protein in the MM fraction exceeded 7-10-fold the protein content in the same fraction of donor blood serum. Hemodialysis decreased dissimilarly the MM concentration in blood serum of various patients, but the content of this fraction was decreased approximately by 37% after the treatment; the constant value was obtained as a result of calculation per an hour of hemodialysis and per kg of body mass. These data suggest that elimination of the MM fraction from blood serum of patients with chronic kidney insufficiency depends on the period of the short-term hemodialysis.