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Immobilization of cytochrome P-450 from rabbit liver microsomes on albumin modified Sepharose 2B

Eremin, A.N.; Metelitsa, D.I.; Usanov, S.A.; Akhrem, A.A.

Prikladnaia Biokhimiia i Mikrobiologiia 16(2): 206-211

1980

ISSN/ISBN: 0555-1099
PMID: 7384010
Document Number: 158121
A highly purified cytochrome P-450 from rabbit liver microsomes has been immobilized on albumin modified omega-aminohexyl-Sepharose 2B, after glutaraldehyde treatment of the matrix. The effect of the degree of albumin modification of the matrix on the catalytic activity of immobolized cytochrome P-450 has been studied in cumene hydroperoxide dependent aniline hydroxylation. On this basis an optimal cytochrome P-450/albumin ratio has been found which allows a substantial decrease in the rate of cytochrome P-450 destruction by cumene hydroperoxide in the substrate absence.

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Johansson, I.; Ingelman-Sundberg, M. 1988: Benzene metabolism by ethanol-, acetone-, and benzene-inducible cytochrome P-450 (IIE1) in rat and rabbit liver microsomes Cancer Research 48(19): 5387-5390
Ohmori, S.; Shirakawa, C.; Motohashi, K.; Yoshida, H.; Abe, H.; Nakamura, T.; Horie, T.; Kitagawa, H.; Asaoka, K.; Rikihisa, T. 1993: Purification from liver microsomes from untreated cynomolgus monkeys of cytochrome P450 closely related to human cytochrome P450 2B6 Molecular Pharmacology 43(2): 183-190
Fisher, A.B.; Huber, G.A.; Furia, L. 1977: Cytochrome P-450 content and mixed-function oxidation by microsomes from rabbit alveolar macrophages Journal of Laboratory and Clinical Medicine 90(1): 101-108
Lü, J.; Xie, G.; Cui, T.; Xu, L. 1998: Immobilization of rat liver microsomes with liquid lipid membrane encapsulation Wei Sheng Yan Jiu 27(3): 149-150
Anzenbacher, P.; Soucek, P.; Anzenbacherová, E.; Gut, I.; Hrubý, K.; Svoboda, Z.; Kvĕtina, J. 1998: Presence and activity of cytochrome P450 isoforms in minipig liver microsomes. Comparison with human liver samples Drug Metabolism and Disposition: the Biological Fate of Chemicals 26(1): 56-59
Clement, B.; Jung, F.; Pfunder, H. 1993: N-hydroxylation of benzamidine to benzamidoxime by a reconstituted cytochrome P-450 oxidase system from rabbit liver: involvement of cytochrome P-450 IIC3 Molecular Pharmacology 43(3): 335-342
Rud'ko, N.P.; Davydov, V.V. 2001: Reductase activity in the liver microsomes in adult and old rats during immobilization stress Voprosy Meditsinskoi Khimii 47(5): 506-510
Shkumatov, V.M.; Gilevich, S.N.; Chashchin, V.L.; Akhrem, A.A. 1983: The C27-steroid hydroxylating system from bovine liver mitochondria. Isolation of ferredoxin (hepatoredoxin) by affinity chromatography on cytochrome-c-sepharose Bioorganicheskaia Khimiia 9(9): 1231-1236
Akhrem, A.A.; Popova, E.M.; Metelitsa, D.I. 1978: Interaction of aliphatid alcohols with cytochrome P-450 from rat liver microsomes Biokhimiia 43(8): 1485-1491
Shimada, T.; Guengerich, F.P. 1990: Inactivation of 1,3-, 1,6-, and 1,8-dinitropyrene by cytochrome P-450 enzymes in human and rat liver microsomes Cancer Research 50(7): 2036-2043
Andries, M.J.; Lucier, G.W.; Goldstein, J.; Thompson, C.L. 1990: Involvement of cytochrome P-450c in alpha-naphthoflavone metabolism by rat liver microsomes Molecular Pharmacology 37(6): 990-995
Shan, W.; Zhang, Y.; Zhu, J.; Shao, Q.; Fan, X. 2012: Inhibitory effects of astragaloside IV on cytochrome P450 enzyme of rat liver microsomes Zhongguo Zhong Yao Za Zhi 37(1): 85-88
Yamazaki, H.; Guo, Z.; Persmark, M.; Mimura, M.; Inoue, K.; Guengerich, F.P.; Shimada, T. 1994: Bufuralol hydroxylation by cytochrome P450 2D6 and 1A2 enzymes in human liver microsomes Molecular Pharmacology 46(3): 568-577
Ozaki, S. 1974: Effect of lipids on liver microsomal membranes. (1) Studies of a relationship between delipidation of liver microsomes with the various organic solvents and cytochrome c reductase activity linked to reduced nicotinamide adenine dinucleotide Hokkaido Journal of Medical Science 49(4): 326-336
Krŭstev, S.; Malashoti, S.; Dancheva, K. 1983: Effect of glucocorticosteroids on exogenous cytochrome C binding by the microsomes and mitochondria isolated from the rat liver Eksperimentalna Meditsina i Morfologiia 22(3): 6-13
Subrahmanyam, V.; Renwick, A.B.; Walters, D.G.; Young, P.J.; Price, R.J.; Tonelli, A.P.; Lake, B.G. 2001: Identification of cytochrome P-450 isoforms responsible for cis-tramadol metabolism in human liver microsomes Drug Metabolism and Disposition: the Biological Fate of Chemicals 29(8): 1146-1155
Mitani, F.; Alvares, A.P.; Sassa, S.; Kappas, A. 1971: Preparation and properties of a solubilized form of cytochrome P-450 from chick embryo liver microsomes Molecular Pharmacology 7(3): 280-292
Ichikawa, M.; Ichikawa, Y. 1979: Some properties and components of an NADH-dependent cytochrome P-450 oxidase system in liver microsomes Medical Journal of Osaka University 30(1-2): 39-56
Fuhr, U.; Wolff, T.; Harder, S.; Schymanski, P.; Staib, A.H. 1990: Quinolone inhibition of cytochrome P-450-dependent caffeine metabolism in human liver microsomes Drug Metabolism and Disposition: the Biological Fate of Chemicals 18(6): 1005-1010
Danie, W.A.; Syrek, M.; Ryłko, Z.; Wójcikowski, J. 2001: Effects of antidepressant drugs on the activity of cytochrome P-450 measured by caffeine oxidation in rat liver microsomes Polish Journal of Pharmacology 53(4): 351-357