Analysis of adipimidate-crosslinked lysine
Ohara, O.; Takahashi, S.
Analytical Biochemistry 107(2): 314-317
1980
ISSN/ISBN: 0003-2697 PMID: 6776844 Document Number: 157060
The chromatographic conditions for separation of N,N'-bislysyl(.epsilon.-N)adipamidine and N-lysyl(.epsilon.-N)adipamidinic acid, which were the products of acid hydrolysis of proteins treated with adipimidate esters, from other amino acids on an amino acid analyzer were established including their ninhydrin color values. Kinetics of deceomposition of these lysine derivatives under the conditions of total acid hydrolysis of protein are reported.
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