Histochemistry of mucosubstances. Technical considerations
Pagnini, C.A.; Bozzola, L.
Annales d'Anatomie Pathologique 25(3): 261-265
1980
ISSN/ISBN: 0003-3871 PMID: 6165260 Document Number: 155325
A report concerning the histochemical methods used to differentiate mucosubstances and certain technical modifications made with the aim of obtaining better results : 1) rapid passage in a solution at pH1 after alcian blue reaction in order to ensure staining of sulfate mucosubstances only ; 20 plunging of sections first in FeCl for 10 minutes by the H.I.D. method ; 3) in the methylation reaction the optimum time is 12 hours.