Dansylcadaverine specific staining for transamidating enzymes
Lorand, L.; Siefring, G.E.; Tong, Y.S.; Bruner-Lorand, J.; Gray, A.J.
Analytical Biochemistry 93(2): 453-458
1979
ISSN/ISBN: 0003-2697 PMID: 88908 Document Number: 152774
Versatile fluorescent staining methodologies, based on the incorporation of dansylcadaverine [N-(5-aminopentyl)-5-dimethylamino-1-naphthalenesulfonamide] into N,N-dimethylcasein, are described for the detection of transamidating enzymes of the endo-.gamma.-glutamine:.epsilon.-lysine transferase type. Activity staining was employed for comparing the electrophoretic behaviors of such transamidating enzymes derived from human and guinea pig tissues. Two enzymatically active forms of guinea pig liver transglutaminase were found.