Fistula-free method of evaluating cholagogue agents on rats
Litvinchuk, M.D.; Rudyĭ, R.V.; Novosilets, Z.I.
Farmakologiia i Toksikologiia 42(6): 674-676
1979
ISSN/ISBN: 0014-8318 PMID: 115710 Document Number: 152274
A new fistula-free method for standardizing and studying the choleretic activity of drugs on white rats is proposed. In rats under ether-urethane anestheia, a "biological test tube" of varied length where the bile is excreted, is created by ligation of the duodenum above and below the site of communication with the bile duct. In order to recover the patency of the digestive tract, the stomach or the proximal segment of the intestine is connected to its distal strip by a polyethylene tube. Supply of pancreatic juice to the "biological test tube" is eliminated by ligation of the pancreatic gland duct. The amount of bile (mg) excreted during a certain period of time, is established from the weight difference between the filled and bile-free dissected isolated segment of the duodenum. The choleretic activity of the drug is assessed in a similar way. The trial of the method performed on 50 rats, with the use of choleretic test-preparations (insulin and mebethisol) confirmed its sensitivty. The method proposed can be recommended for preliminary screening and dynamic study of the choleretic activity of drugs.