Intravital detection of the cellular outer perimembrane layers (glycocalyx) using alcian blue. II. the effect of trypsin processing on glycocalyx sorption of alcian blue
Krylenkov, V.A.; Levin, S.V.; Samoĭlova, K.A.
Tsitologiia 21(4): 470-473
1979
ISSN/ISBN: 0041-3771 PMID: 88106 Document Number: 151959
Trypsin treatment of Zajdela ascitic hepatoma cells results in destruction of outer perimembrane layers (glycocalyx), which is manifested in release of their proteins and carbohydrates. The process is accompanied by reduction of alcian blue sorption by the cell surface and decrease of cell viability.