Oxidation of fatty-aromatic aldehydes in liver tissues
Kholmina, G.V.; Gorkin, V.Z.
Voprosy Meditsinskoi Khimii 25(3): 322-328
1979
ISSN/ISBN: 0042-8809 PMID: 36712 Document Number: 151423
A protein fraction, which did not contain NADP- or NADPH-dependent aldehyde reductases and NAD- or NADP-dependent aldehyde dehydrogenases, but which catalyzed oxidation of fatty-aromatic aldehydes, was isolated from extract of rat liver tissue using ammonium sulfate fractionation combined with chromatography on DEAE-Sephadex A-25 or Molselect DEAE-25, CM-Sephadex C-25 and gel filtration on Sephadex G-200. Investigations of MW and catalytic properties of the protein fraction obtained enabled identification of it with xanthine oxidase [EC 1.2.3.2]. Aldehyde dehydrogenases and xanthine oxidase are involved in oxidation of fatty-aromatic aldehydes to corresponding fatty acids, besides the reduction of the aldehydes to alcohols, catalyzed by aldehyde reductases and alcohol dehydrogenases.