Isolation, structural organization and mechanism of action of mitochondrial steroid hydroxylating systems
Akhrem, A.A.; Lapko, V.N.; Lapko, A.G.; Shkumatov, V.M.; Chashchin, V.L.
Acta Biologica et Medica Germanica 38(2-3): 257-273
1979
ISSN/ISBN: 0001-5318 PMID: 517002 Document Number: 144970
A scheme for the isolation of individual proteins of the 20S,22R-cholesterol hydroxylating system is presented. Physico-chemical and structural parameters of cytochrome P-450 are furnished. Self-reconstitution of indivudal proteins of the system into an enzyme unit is shown. Furthermore a regulation process of electron transport in the 20S,22R-cholesterol system is considered.