Assay of sulfotransferases
Sekura, R.D.; Marcus, C.J.; Lyon, E.S.; Jakoby, W.B.
Analytical Biochemistry 95(1): 82-86
1979
ISSN/ISBN: 0003-2697 PMID: 495970 Document Number: 144146
Two methods are described for the assay of sulfotransferases which are active with sulfate acceptors bearing the hydroxyl functional group. Assays were developed for enzymes which transfer sulfate from 3'-phosphoadenosine-5'-phosphosulfate (PAPS) to sterols, phenols and simple alcohols, thereby forming the corresponding sulfate esters. With a filter binding assay, useful with crude and purified PAPS is used and the labeled product is separated from PAPS and inorganic sulfate by a TLC technique in which the product migrates close to the solvent front; the assay is useful with a broad array of substrates and is more sensitive than the filter binding assay.