Presence of Murine Leukemia Virus (MLV) -related Virus Gene Sequences in a Commercial RT-PCR Reagent
Wolff, D.; Gerritzen, A.
Clinical Laboratory 57(7-8): 631-634
2011
ISSN/ISBN: 1433-6510 PMID: 21888029 Document Number: 14228
Background: The recent identification of murine leukemia virus (MLV)-related viruses in patients with chronic fatigue syndrome (CFS) has aroused much interest, not least among sufferers. However, other studies failed to detect these viruses in CFS patients. Methods: We wanted to establish a MLV-related virus real-time PCR for routine diagnostics. Results: Our study identified false positive MLV-related virus results due to a contamination of Superscript III Platinum One-Step Quantitative RT-PCR System (Invitrogen). Conclusions: This observation may be helpful to elucidate discrepant results for the detection of MLV-related virus like xenotropic MLV-related virus (XMRV) in recently published studies.
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