Prediction of Bladder Cancer Based on Urinary Content of MGEA5 and OGT mRNA Level
Rozanski, W.; Krzeslak, A.; Forma, E.; Brys, M.; Blewniewski, M.; Wozniak, P.; Lipinski, M.
Clinical Laboratory 58(5-6): 579-583
2012
ISSN/ISBN: 1433-6510 PMID: 22783592 Document Number: 14181
Background: The mRNA expression of genes coding enzymes involved in O-GlcNAcylation were analyzed in urine obtained from 176 bladder cancer (BC) patients and 143 healthy persons. Methods: MGEA5 and OGT expression was measured by a real-time PCR assay. Results: OGT expression was not detected in urine of healthy persons but it was found in 51.7% of BC samples. Positive expression of MGEA5 was found in urine of both healthy persons (47.1%) and BC patients (52.3%). Poorly differentiated BC (grade III) showed significantly lower MGEA5 expression than grade I tumors. Contrary, OGT transcript level was significantly higher in grade II and III in comparison to grade I BC. Moreover, there was significant difference in OGT expression between early bladder cancers and invasive or advanced bladder cancers. Conclussions: These results suggest that analysis of urinary content of MGEA5 and OGT may be useful for bladder cancer diagnostics.
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