Original removing technique of the kidneys for transplantation. An experimental study
Barra, J.A.; Kermarrec, J.Y.; Clèdes, J.; Leroy, J.P.; Le Saout, J.
Journal de Chirurgie 116(3): 225-230
1979
ISSN/ISBN: 0021-7697 PMID: 385608 Document Number: 140448
The depicted procedure minimizes the hazards of aggressive manipulations, and excludes any possibility of so-called "warm ischaemia". The kidneys are flushed out in situ with cool pulsatile perfusion through an aortic cannula. Kidneys, aorta and inferior vena cava are then removed en-bloc without interrupting the perfusion, and preserved in Collins' solution at 4 degrees C. After a 6 to 19 hour storage time, one of the kidneys is transplanted on another dog, while the second one is submitted to histological examination. Nine transplantations were carried out, followed in 7 cases with a most satisfactory diuresis resuming, and with urinary ionic levels, as well as daily urea output, normalization within 48 hours. Histologic examination showed an unaffected glomerular apparatus, but some mild tubular lesions.