Use of Vaccinia virus as an adjuvant in inducing specific anti-tumour immunity to transplantable mouse fibrosarcoma. I. Adaptation of the virus to mouse tumour cells as a pre-requisit for adjuvanticity
Bendre, A.R.; Gangal, S.G.
Indian Journal of Cancer 15(4): 1-8
1978
ISSN/ISBN: 0019-509X PMID: 757683 Document Number: 138706
Experiments were carried out to test the possibility of using vaccinia virus as an immunoadjuvant to induce antitumor immunity in a mouse fibrosarcoma mode. The ability of vaccinia virus to infect and multiply in primary and established cultures of a transplantable mouse fibrosarcoma (MFS) was studied. MFS cells were infected with a known dilution of vaccinia virus for 18-24 h. The multiplicity of virus was assayed by studying virus pock formation on chick chorioallantoic membrane. The virus multiplied 4-16 times in MFS cells without destroying the cells. Typical viral inclusion bodies were seen in the cytoplasm of infected cells. Experiments carried out to see surface alterations of the infected cells revealed that the ability of infected cells to absorb specific isoantibodies was reduced when compared with non-infected cells. Vaccinia virus fulfils the prerequisites necessary for being a potent immunoadjuvant in inducing antitumor immunity.