Myristica fragrans Houtt. methanolic extract induces apoptosis in a human leukemia cell line through SIRT1 mRNA downregulation
Chirathaworn, C.; Kongcharoensuntorn, W.; Dechdoungchan, T.; Lowanitchapat, A.; Sa-nguanmoo, P.; Poovorawan, Y.
Journal of the Medical Association of Thailand 90(11): 2422-2428
2007
ISSN/ISBN: 0125-2208 PMID: 18181330 Document Number: 13660
Myristica fragrans Houtt. (nutmeg) contains antibacterial, antiviral and anti-cancer activities. However the mechanisms underlying those activities have not been clearly explained. To study the effect of Myristica fragrans Houtt. methanolic extract on Jurkat human leukemia T cell line. Methanol extract of Myristica fragrans Houtt. (Myristicaceae) was used to study the effect on Jurkat cell metabolic activity using an MTT assay and on apoptosis using annexin V staining. Expression of SIRT1 gene was determined by RT-PCR. At the concentrations 50 and 100 ig/mL, the methanol extract of Myristica fragrans Houtt significantly inhibited Jurkat cell proliferation and induced apoptosis as detected by annexin V staining. Downregulation of SIRT1 mRNA expression in Jurkat cells was observed even when the amount of methanol extract was 10 microg/mL. Methanol extract of Myristica fragrans Houtt induced apoptosis of Jurkat leukemia T cell line in a mechanisms involving SIRTI mRNA downregulation.
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