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Episomal viral DNA in herpesvirus saimiri-transformed lymphoid cell lines

Werner, F.J.; Bornkamm, G.W.; Fleckenstein, B.; Mulder, C.

Iarc Scientific Publications 1978(24 Part 1): 125-130

1978

ISSN/ISBN: 0300-5038
PMID: 221324
Document Number: 133074
Structural analysis of episomal viral genomes from two herpesvirus saimiri (HVS)-transformed tumour cell lines (No. 1670 and 70N2) showed that both types of episomes have a higher molecular weight than linear virion DNA. The arrangement of unique (L) and repetitive (H) DNA in No. 1670 episomes was studied by partial denaturation mapping. Part of the L-sequences present in linear virion DNA was found to be missing, part was found to be duplicated in the episomes. The episomal L-DNA regions were correlated with the known physical gene maps of linear HVS DNA.

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