A new approach to microhomogenization
Hinegardner, R.; Alderton, J.
Analytical Biochemistry 85(2): 401-403
1978
ISSN/ISBN: 0003-2697 PMID: 646096 Document Number: 133030
A versatile microhomogenizer is described which is capable of handling sample volumes ranging from 1 .mu.l to several milliliters. The sample and homogenization buffer are sealed within a segment of flexible plastic tubing. The sample is disrupted by rubbing or rolling a metal rod back and forth over the length of tubing. Centrifugation of the homogenate is performed in the same sealed tubing segment. This method can be used to homogenize single cells.
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