Measurement of the horny layer turnover after staining with dansyl chloride: description of a new method
Johannesson, A.; Hammar, H.
Acta Dermato-Venereologica 58(1): 76-79
1978
ISSN/ISBN: 0001-5555 PMID: 75640 Document Number: 132864
A simple nonradioactive technique is described. The corneal layers were stained with 5% dansyl chloride ointment during 24-48 h of occlusion. The number of newly formed corneal layers were counted on sections from punch biopsies obtained at various intervals after the staining. The rate of formation is given as layers formed per 24 h. The technique makes it possible to detect an increase in the turnover rate when it is more than 15-32% above normal. The biological material can also be utilized for comparative studies utilizing biochemical and histological methods. The average rate of formation was 1.15 .+-. 0.09 (S.E.M. ) layers/24 h in a group of 10 subjects.