A chromatographic method for the urinary pregnanediol dosage for routine analyses
Castronuovo, A.; Antonucci, M.; Di Renzo, M.; Ricci, E.
Quaderni Sclavo di Diagnostica Clinica E di Laboratorio 15(3): 395-401
1978
ISSN/ISBN: 0033-4979 PMID: 752831 Document Number: 132789
The AA. aiming at a handy and speedy system admitting of being fitted to the equipments of any laboratory hospital have taken into account two procedures, one proposed by Varley and the other of Fotherby and Love reported by Wootton and King for pregnanediol. The two methods have been combined in order to make the process easier. The procedure is based upon chromatography on alumina of ethereal extracts dissolved in cyclohexan. Glucuronate hydrolysis has been obtained with beta-glucuronidase.