Method of determining testosterone in the blood on a Tsvet-104 gas chromatograph with a constant recombination rate detector
Bogdarin, I.A.
Problemy Endokrinologii 24(5): 48-52
1978
ISSN/ISBN: 0375-9660 PMID: 704556 Document Number: 132453
The author suggests a method of blood testosterone level determination on gas chromatograph "Tsvet-104" with a constant recombination rate detector, without any use of radioactive isotopes, testosterone derivatives. The method consists in thorough preliminary extraction of endogenous testosterone from the blood, its purification in thin layers on silicalgel, and conversion into a fluoroacetoderivative with a subsequent gaseochromatographic separation on chromatone N-AW-DMCS (60--80 mesh) with a low (5%) SE-30 phase content at high temeprature regimens of gas chromatograph "Tsvet-104" with a high resolving power of its selective detector.