Determination of fluoroacetate in biological matrixes as the dodecyl ester
Burke, D.G.; Lew, D.K.; Cominos, X.
Journal - Association of Official Analytical Chemists 72(3): 503-507
1989
ISSN/ISBN: 0004-5756 PMID: 2745378 Document Number: 131
A new method for the quantitative determination of fluoroacetate in biological samples was applied to a number of avian samples. Fluoroacetate is isolated as its potassium salt by ion-exchange chromatography and directly converted to its dodecyl ester, using a novel derivatization procedure. The ester is measured by capillary gas chromatography with a flame ionization detector for the range 1.0-10.0 micro g/g and by selected ion monitoring GC/mass spectrometry for the range 0.01-1.00 micro g/g. Recoveries from 1 g chicken muscle were about 80%. The method was applied to the determination of fluoroacetate in the crop, stomach, liver, heart, intestine, and breast muscle of 5 zebra finches (Peophila guttata) that had been fed millet containing 9 micro g/g of sodium fluoroacetate. Despite a wide variation in dose, the levels in organs and tissues were approximately 1 micro g/g, except for heart tissue which was about 2 micro g/g. The presence of interfering peaks at low levels necessitated the use of selected ion monitoring GC/MS when sample weights were less than 1 g or when levels were less than 1 micro g/g. Samples can be analyzed within hours of receipt; therefore, the method is suitable for routine use in a diagnostic laboratory.
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