Histochemical type I fibres in the soleus of the rat
Dekleva, A.; Sirca, A.
Journal of Anatomy 127(Pt 3): 595-601
1978
ISSN/ISBN: 0021-8782 PMID: 154494 Document Number: 126529
Based on oxidative enzyme activity levels, fibers exhibiting moderate and high levels may be identified in the rat soleus. Fibers showing moderate activity are classified as type I fibers, while those showing high activity may belong to type I or type II. According to the level of ATPase activity in fixed sections, 3 types of fibers can be distinguished in the rat soleus (IA, IB and II) and, by application of acid preincubation, also sub-classes of type II (IIA and IIC). Type IB fibers possess high oxidative and glycolytic enzyme activities, moderate ATPase activity after fixation, and behave in the same way as type I fibers after alkaline and acid preincubation. For the histochemical classification of fiber types, reactions to ATPase should be condsidered after acid preincubation, and also reactions to the enzymes of oxidative and glycolytic metabolism.