Top Abstract Purchase Related
Home
>
Node
>
Subnode

Isolation and characterization of a cytosol protein (64/7.2) present in large amounts in rapidly growing hepatomas

Raju, K.S.; Cartwright, A.; Hirsch, F.W.; Wu, B.C.; Nall, K.N.; Morris, H.P.; Busch, H.

Cancer Research 38(7): 1922-1928

1978

ISSN/ISBN: 0008-5472
PMID: 207417
Document Number: 126096
Protein 64/7.2 (molecular weight/isoelectric point) is present in the cytosol of several hepatomas including the Novikoff hepatoma and Morris hepatomas 9618A, 7794A, and 3924A, but it is not present in liver or 18-hr regenerating liver. Quantitatively, its concentration was highest in Novikoff hepatoma (150 microgram/g tissue) and Morris hepatoma 3924A (550 microgram/g tissue), which are rapid-growing tumors, less in Morris hepatoma 7794A (72 microgram/g tissue), which is of intermediate growth rate, and least in the slow-growing Morris hepatoma 9618A (25 microgram/g tissue). Protein 64/7.2 was isolated from Novikoff hepatoma ascites cells in high purity as shown by its migration as a single band on one-dimensional acid-urea-polyacrylamide gels and a single spot on two-dimensional isoelectric focusing sodium dodecyl sulfate-polyacrylamide gels. Its amino acid composition has an acidic to basic amino acid ratio of 1.6. Its amino-terminal amino acid is lysine, and its carboxyl-terminal amino acid is glycine. Interestingly, the amino acid composition is strikingly similar to that of the phosphorylated Novikoff hepatoma chromatin Protein Cg', partially characterized in our laboratory.

Document emailed within 1 workday
Secure & encrypted payments

Raju, K.S.; Morris, H.P.; Busch, H. 1980: Purification and characterization of cytosol protein 45/7.8 present in rapidly growing hepatomas Cancer Research 40(5): 1623-1629
Senjo, M. 1985: Isolation and characterization of heme transfer protein involved in biosynthesis of microsomal cytochrome b5 from rat liver cytosol Hokkaido Journal of Medical Science 60(5): 625-634
Esteban, J.; Gutiérrez, F.; Fariña, M.C.; Martín-Moreno, L.; Requena, L.; Jiménez-Arriero, M.; Soriano, F. 1997: Clinical significance of the isolation of rapidly growing mycobacteria Enfermedades Infecciosas y Microbiologia Clinica 15(5): 260-263
Schiller, C.M.; Huang, T.I.; Heizer, W.D. 1977: Isolation and characterization of four peptide hydrolases from the cytosol of rat intestinal mucosa Gastroenterology 72(1): 93-100
Aĭtkhozhin, M.A.; Azimuratova, R.Z.; Kim, T.N.; Darkanbaev, T.B. 1972: Isolation and characterization of rapidly labelled fractions of cytoplasmic RNA of Aspergillus niger Biokhimiia 37(6): 1276-1281
Moodycliffe, A.M.; Maiti, S.; Ullrich, S.E. 1999: Splenic NK1.1-negative, TCR alpha beta intermediate CD4+ T cells exist in naive NK1.1 allelic positive and negative mice, with the capacity to rapidly secrete large amounts of IL-4 and IFN-gamma upon primary TCR stimulation Journal of Immunology 162(9): 5156-5163
Roth, J.S.; Hsieh, L.L.; Peraino, C.; Weinstein, I.B. 1990: Isolation of a complementary DNA encoding the catalytic subunit of protein kinase A and studies on the expression of this sequence in rat hepatomas and regenerating liver Cancer Research 50(6): 1675-1680
Li, T.; Zhou, J.; Xu, Z.; Pan, J.; Mao, S. 1990: Effects of processing methods on the amounts of volatile oil of nutmeg and on isolation and characterization of the volatile oil constituents Zhongguo Zhong Yao Za Zhi 15(7): 405-407; 446
Meijer-Huizinga, F.; Mertens, K.; van Mourik, J.A. 1994: Isolation and characterization of single-chain protein S Thrombosis and Haemostasis 72(3): 408-414
Alunni, A.; Vaccari, S.; Torcia, S.; Meomartini, M.E.; Nicotra, A.; Alfei, L. 2005: Characterization of glial fibrillary acidic protein and astroglial architecture in the brain of a continuously growing fish, the rainbow trout European Journal of Histochemistry: Ejh 49(2): 157-166
Takiguchi, M.; Fujinaga, T.; Naiki, M.; Mizuno, S.; Otomo, K. 1990: Isolation, characterization, and quantitative analysis of C-reactive protein from horses American Journal of Veterinary Research 51(8): 1215-1220
Rosenthal, C.J.; Franklin, E.C.; Frangione, B.; Greenspan, J. 1976: Isolation and partial characterization of SAA-an amyloid-related protein from human serum Journal of Immunology 116(5): 1415-1418
Marzona, M. 1974: Structure of the keratin of psoriasic squamae. II. Isolation and characterization of a pure protein component Bollettino della Societa Italiana di Biologia Sperimentale 50(20): 1658-1661
Inoue, M. 1978: Studies on guanosine 3' :5'-monophosphate-dependent protein kinase; isolation and characterization of catalytic fragment Kobe Journal of Medical Sciences 24(3): 125-140
Fujii, T.; Nakamura, T.; Sekizawa, A.; Tomonaga, S. 1992: Isolation and characterization of a protein from hagfish serum that is homologous to the third component of the mammalian complement system Journal of Immunology 148(1): 117-123
Davies, D.A.; Kalinina, N.A.; Samokhvalova, L.V.; Malakhova, G.V.; Scott, G.; Volynskaia, A.M.; Nesmeianov, V.A. 2005: Isolation and characterization of the ALP1 protease from Aspergillus fumigatus and its protein inhibitor from Physarium polycephalum Bioorganicheskaia Khimiia 31(3): 259-268
Harview, C.L.; McKenzie, S.A.; Hsiao, J.L. 2019: Rapidly growing lesions on the forehead Journal of Family Practice 68(5): 293-295
Solovev, A.A.; Churunov, V.A.; Erokhin, I.E. 1977: Isolation and characterization of pigment-protein complexes from green serobacteria Chlorobium limicola forma thiosulfatophilum Biokhimiia 42(9): 1697-1703
Premkumar, E.; Bhargava, P.M. 1973: Isolation and characterization of newly synthesized RNA and protein in mature bovine spermatozoa and effect of inhibitors on these syntheses Indian Journal of Biochemistry and Biophysics 10(4): 239-253
Cardis, M.A.; Huppmann, A.R.; Kirkorian, A.Y. 2018: Rapidly growing subcutaneous mass in an infant Dermatology Online Journal 24(5)