Cholinesterase assay by gas--solid chromatography
Somorin, O.
Analytical Biochemistry 88(2): 442-448
1978
ISSN/ISBN: 0003-2697 PMID: 29518 Document Number: 125644
The determination of serum cholinesterase activity was based on the hydrolysis of butyrylcholine chloride, solvent extraction, and the quantitative estimation of the hydrolytic product, butyric acid, by gas solid chromatography. Using this procedure, optimum conditions of pH and buffer strength for the cholinesterase activity were pH 8.0 and 0.05 M Tris-HCl, respectively. The cholinesterase activities in blood samples from healthy subjects, as determined by this method, were in the range of normal values reported in the literature. The effects of eserine and other drugs on the cholinesterase activity were also reported. The high accuracy (1.1%) and high sensitivity which permit the determination of butyric acid at levels down to 1 .mu.g/ml make this procedure attractive as a serum cholinesterase assay.