Immunomorphologic study of the early stages of amyloidogenesis
Kapinus, L.N.
Biulleten' Eksperimental'noi Biologii i Meditsiny 85(2): 232-234
1978
ISSN/ISBN: 0365-9615 PMID: 343835 Document Number: 125120
Early stages of amyloidogenesis were studied in mice with experimental amyloidosis. According to indirect Coons' method the cryostat sections of the spleens and other organs of mice were incubated first with pure rabbit fibrillar protein antiamyloid antibodies, and then with pure fluorescein-labeled foat antirabbit IgG antibodies. At the early period after 1--2 casein injections there appeared amyloid protein in the intercellular space and in the blood filling the spleen and liver sinuses. Formation of the typical amyloid deposits in the spleen were completed by the 5th--7th day of the experiment after 3--4 casein injections.