The comparison of viral antibody titers of acid-precipitated and non-precipitated mouse ascitic fluids
Gamble, W.C.; Chappell, W.A.; George, E.H.
Health Laboratory Science 15(2): 91-94
1978
ISSN/ISBN: 0017-9035 PMID: 681182 Document Number: 124394
Mouse immune ascitic fluid has become a primary source of antibody for diagnostic, reference, and research work in many virus laboratories. The inherent disadvantage of ascitic fluid is that it repeatedly forms clots and subsequently loses volume. The acid-precipitation method of Chiewsilp and McCown eliminates the clot formation and does not appreciably alter the antibody titers for several arboviruses, varicella, rabies and influenza viruses.