A simplified method for selective silver impregnation of degenerating axons and their terminals in the central nervous system
Yamadori, T.
Kobe Journal of Medical Sciences 23(1-4): 1-5
1977
ISSN/ISBN: 0023-2513 PMID: 77935 Document Number: 123137
A simplified method for selective Ag impregnation of degenerating axons and their terminals was developed, modifying the Nauta-Gygax method and was used in experiments on the fiber connection in the CNS of mice, rats and dogs with satisfactory results. An EM observation was also performed to confirm the result. The main merits of this method are: the stability of the result, always producing dark, and dot-like degenerating axons and their terminals against a clean, light-golden background; and the very much shortened time of the whole procedure which enables the staining of a large number of sections in 30-40 min.