The interference by erythrocyte "acetylthiocholinesterase" in the estimation of the blood cholinesterase activity of the chicken
Pickering, C.E.; Pickering, R.G.
Toxicology and Applied Pharmacology 39(2): 229-237
1977
ISSN/ISBN: 0041-008X PMID: 847753 Document Number: 122810
In deducing possible exposure of chickens to organophosphorus compounds from the measurement of blood cholinesterase activity, it is preferable to use plasma separated from the whole blood rather than the whole blood. Whole blood must not be used in methods which involve hemolysis and use acetylthiocholine as substrate. This is because there is acetylthiocholinesterase but not acetylcholinesterase activity within the chicken erythrocytes. When only small samples of unhemolyzed whole blood are available (e.g., under field conditions), it is possible to measure plasma cholinesterase activity by the Voss and Sachsse method, which avoids hemolysis, but does use acetylthiocholine as substrate.