Noncytotoxic IgE-mediated release of histamine and serotonin from murine mastocytoma cells
Taurog, J.D.; Mendoza, G.R.; Hook, W.A.; Siraganian, R.P.; Metzger, H.
Journal of Immunology 119(5): 1757-1761
1977
ISSN/ISBN: 0022-1767 PMID: 72105 Document Number: 122759
Cultured murine mastocytoma (AB-CBF1-MCT-1) cells were stimulated to release endogenous or incorporated histamine or serotonin by an IgE-mediated mechanisms without loss of viability. Stimulation was achieved by incubation of the cells with rat IgE-anti-IgE, rat IgE-anti-light chain, fluoresceinated rat IgE-anti-fluorescein, IgE-enriched mouse anti-ovalbumin-ovalbumin, or covalently linked dimers of rat IgE, at doses similar to those optimal for normal peritoneal mast cells. Active cell metabolism and Ca++ were required to obtain release. Despite the latter, no dose of the calcium ionophore, A23187, could be found which caused release without concomitant cytotoxicity. Phosphatidylserine did not enhance release.