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Isolation of T-lymphocytes from disaggregated tumors, with high purity and good percentage recovery

Hansen, C.B.; Gillespie, G.Y.; Russell, S.W.

Journal of the National Cancer Institute 59(1): 273-275

1977

ISSN/ISBN: 0027-8874
PMID: 195071
Document Number: 122445
A combination of two cell separation methods was utilized for the isolation of thymus-derived lymphocytes (TL) from enzymatically disaggregated tumors. Passage through Sephadex G-10-glass bead columns to remove adherent cell types followed by exposure to IgG-coated sheep red blood cell monolayers for removal of Fc receptor-bearing inflammatory cells provided functional TL suspensions of high purity with good percentage recovery.

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