Comparison of Two Different Screening Methods for the KRAS Mutation in Colorectal Cancer

Tze-Er, K.; Ya-Chang, S.; Kun-Yeh, T.; Tien-Chang, J.; Jan-Gowth Chang

Clinical Laboratory 56(5-6): 175-186

2010


ISSN/ISBN: 1433-6510
PMID: 20575464
Document Number: 11730
Background: To compare high resolution melting (HRM) and primer extension (PE) for mutation screening of KRAS codons 12 and 13. Methods: DNA samples were isolated from 60 colorectal cancer (CRC) patients. HRM and primer extension analyses were used for mutation screening of KRAS codons 12 and 13. Results: Both methods can detect KRAS mutations in a DNA mixture containing as little as 5 % mutant DNA. The concordant rate between the two methods was 100 %. HRM analysis is able to distinguish mutant from wild type, however, it is unable to detect the actual base change. PE analysis needs more procedures, is time-consuming and slightly more expensive but it is able to detect the precise mutation. Conclusions: This study showed that HRM is a reliable screening method to identify mutations in the KRAS gene, and the PE can be used as a diagnostic method to accurately pinpoint the nature of mutations.

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Comparison of Two Different Screening Methods for the KRAS Mutation in Colorectal Cancer