Top Abstract Purchase Related
Home
>
Node
>
Subnode

Ligand-binding radioassay for the antifolate compounds: application in patients receiving methotrexate

Rothenberg, S.P.; de Costa, M.; Iqbal, M.P.

Cancer Treatment Reports 61(4): 575-584

1977

ISSN/ISBN: 0361-5960
PMID: 884692
Document Number: 114924
The general principles of ligand-binding radioassays are reviewed and a specific noncompetitive system is described for the measurement of methotrexate (MTX) using dihydrofolate reductase as the binding determinant. This type of radioassay can also be exploited to measure any antifolate compound which binds to this enzyme even if [3H]MTX is used as the tracer. The radioassay has now been used to measure MTX in all types of body fluids, tissue extracts, and rbc lysates from patients receiving this drug.

Document emailed within 1 workday
Secure & encrypted payments

Gryzunov, I.A.; Komarova, M.N. 2004: A study of ligand binding to protein using resonance energy transfer from the isoindole fluorescent label to ligand Biofizika 49(6): 979-986
Witty, L.A.; Steiner, F.; Curfman, M.; Webb, D.; Wheat, L.J. 1992: Disseminated histoplasmosis in patients receiving low-dose methotrexate therapy for psoriasis Archives of Dermatology 128(1): 91-93
Ohta, M.; Ohta, K.; Mori, F.; Itoh, N.; Nishitani, H.; Hayashi, K. 1990: Improved radioassay of anti-acetylcholine receptor antibody: application for the detection of extremely low antibody titers in sera from patients with myasthenia gravis Clinical Chemistry 36(6): 911-913
Hansen, S.I.; Holm, J.; Lyngbye, J. 1982: Detergent activation of the binding protein in the folate radioassay Clinical Chemistry 28(1): 117-118
Iqbal, M.P.; Malik, I.; Mehboobali, N. 1992: Appearance of a methotrexate binding plasma protein during high dose methotrexate therapy JPMA. Journal of the Pakistan Medical Association 42(8): 193-196
Eilat, D.; Steinberg, A.D.; Schechter, A.N. 1978: The reaction of SLE antibodies with native, single stranded RNA: radioassay and binding specificities Journal of Immunology 120(2): 550-557
Ithakissios, D.S.; Kubiatowicz, D.O.; Windorski, D.C.; Wicks, J.H. 1977: Binding proteins from fish sera and intrinsic factor compared in vitamin B12 radioassay Clinical Chemistry 23(11): 2043-2047
Leboulenger, F.; Trochard, M.C.; Morin, J.P.; Dupont, W.; Vaudry, H.; Vaillant, R. 1977: Competitive protein-binding radioassay of corticosterone in the plasma of the frog, Rana esculenta L. Journal de Physiologie 73(1): 73-83
Rudorff, K.H.; Herrmann, J.; Kröll, H.J.; Krüskemper, H.L. 1976: Measurement of thyroxine binding globulin by competitive ligand binding assay (CLBA) Journal of Clinical Chemistry and Clinical Biochemistry 14(1): 31-36
Schlegel, H.B.; Poe, M.; Hoogsteen, K. 1981: Models for the binding of methotrexate to Escherichia coli dihydrofolate reductase: direct effect of carboxylate of aspartic acid 27 upon ultraviolet spectrum of methotrexate Molecular Pharmacology 20(1): 154-158
Tu, L.; Chen, A.; Delahunty, M.D.; Moore, K.L.; Watson, S.R.; McEver, R.P.; Tedder, T.F. 1996: L-selectin binds to P-selectin glycoprotein ligand-1 on leukocytes: interactions between the lectin, epidermal growth factor, and consensus repeat domains of the selectins determine ligand binding specificity Journal of Immunology 157(9): 3995-4004
Netteland, B.; Bakke, O.M. 1977: Inadequate sample-preparation technique as a source of error in determination of erythrocyte folate by competitive binding radioassay Clinical Chemistry 23(8): 1505-1506
Buranakitjaroen, P.; Kittipawong, P.; Koanantakul, B.; Piamsomboon, C.; Prasassarakich, N.; Silaraks, S.; Sukonthasarn, A.; Tiyapant, A. 2003: Efficacy and safety of rilmenidine, a selective imidazoline I1 receptor binding ligand, in mild-to-moderate Thai hypertensive patients Journal of the Medical Association of Thailand 86(10): 903-910
Salasoo, S.; Irving, M.G.; Freedman, A. 1976: Methotrexate megadose followed by folate rescue. II. Clearance patterns in patients receiving sequential megadose infusions Medical Journal of Australia 1(22): 826-828
Franklin, T.J. 1983: Energetics of ligand binding to receptors Journal of Neural Transmission. Supplementum 18: 55-60
Wutzen, J. 1990: Histological and histochemical examinations of myocardium of rats kept on low-magnesium diet and receiving methotrexate Materia Medica Polona. Polish Journal of Medicine and Pharmacy 22(3): 156-161
Skvortsov, A.A.; Mareev, V.Y.; Chelmakina, S.M.; Baklanova, N.A.; Belenkov, I.N. 2007: Efficacy and safety of long-term application of spironolactone in patients with moderate and severe chronic heart failure receiving optimal therapy Kardiologiia 47(10): 12-23
Boner, G.; Kristal, H. 1983: Neoplastic disease in patients receiving dialytic therapy and in patients after receiving transplantation Harefuah 104(2): 68-69
Hirsch, J.D.; Beyer, C.F.; Malkowitz, L.; Loullis, C.C.; Blume, A.J. 1989: Characterization of ligand binding to mitochondrial benzodiazepine receptors Molecular Pharmacology 35(1): 164-172
Tuckwell, D.S.; Weston, S.A.; Humphries, M.J. 1993: Integrins: a review of their structure and mechanisms of ligand binding Symposia of the Society for Experimental Biology 47: 107-136