Transplantation in miniature swine. II. in vitro parameters of histocompatibility in MSLA homozygous minipigs
Leight, G.S.; Sachs, D.H.; Rosenberg, S.A.
Transplantation 23(3): 271-276
1977
ISSN/ISBN: 0041-1337 PMID: 140482 Document Number: 114518
A selective breeding program based on serological techniques was used to establish three herds of miniature swine, each homozygous for a different allele at the major histocompatibility complex, termed MSLA. By selective immunization of these swine, high-titered antisera detecting the products of individual MSLA alleles were raised and used to confirm the genotype of offspring in these herds. Techniques for performing one-way mixed leukocyte cultures in these pigs were developed and demonstrated the presence of a strong mixed leukocyte culture locus (or loci) closely linked to the serologically defined MSLA locus. Cell-mediated cytotoxicity assays based on 51Cr release were developed to monitor the course of cellular immunity following allotransplantation and exhibited specificity identical to that predicted by the serological typing.