Effect of somatostatin on growth hormone release by MtT-F4 rat pituitary tumor in vitro
Lis, M.; Motomatsu, T.; Chrétien, M.
Cancer Research 37(8 Part 1): 2612-2614
1977
ISSN/ISBN: 0008-5472 PMID: 194684 Document Number: 114099
Fluoride-stimulated adenylate cyclase is demonstrated inisolated tumor cells of transplantable rat pituitary tumor MtT-F4 in vitro. The intracellular cyclic adenosine 3':5'-monophosphate is lowered in the cells incubated in the presence of synthetic somatostatin. Contrary to the findings reported for normal pituitary, however, the immunoreactive growth hormone release does not change when either somatostatin or phosphodiesterase inhibitors are present in the incubation medium. The presence of dibutyryl cyclic adenosine 3':5'-monophosphate (5 mM) in the incubation medium does not change the rate of growth hormone release by isolated tumor cells.